Introduction and goals
The main theme of the project is to determine the regulators of hematopoietic stem cells (HSC). In this case we are interested in the negative regulators of this process, invastigating it by using shRNA screens.
In this analysis I used the edgeR worfklow to normalize the read counts, and to fit a linear model on it. Apart of this I also fitted a Mixed effects linear model, which also accounts for off-target effects and gene-wise variation in the data.
